荧光显微光谱成像系统及教学设计FLUORESCENCE MICROSCOPIC SPECTRAL IMAGING SYSTEM AND TEACHING DESIGN
刘东奇,鄢小卿,惠王伟,王槿,孔勇发
摘要(Abstract)：
本文介绍了一种自组荧光显微光谱成像系统。该系统性能稳定、功能多样，可以实现空间显微分辨和光谱探测功能的有机结合，具有白光照明成像、激光激发荧光成像、显微光谱成像、荧光光谱探测和应力调控等功能。实验结果表明，该系统的光学分辨率385nm,光谱分辨率小于1nm,可以完成量子点和单层二维材料的荧光光谱探测、白光照明成像和显微光谱成像等。依托该系统设计开展的实验内容充足、层次分明，可满足不同学生的学习需求，为目前国内荧光光谱相关的物理实验教学提供思路。
关键词(KeyWords)： 荧光光谱;显微成像;光谱成像
基金项目(Foundation): 高等学校教学研究项目(DJZW201909hb,DJZW202010hb);; 港澳与内地高等学校师生交流计划项目(202111120070);; 南开大学自制实验教学仪器设备滚动培育项目(2018NKZZYQ25);南开大学物理基地能力提高项目(J1103208);; 国家基础科学人才培养基金项目(J1210027)
作者(Author): 刘东奇,鄢小卿,惠王伟,王槿,孔勇发
参考文献(References)：
- [1] 肖功海，舒嵘，薛永祺.显微高光谱成像系统的设计[J].光学精密工程，2004,12(4):367-372.XIAO G H,SHU R,XUE Y Q.Design of microscopic hyperspectral imaging system[J].Optics and Precision Engineering,2004,12(4):367-372.(in Chinese)
- [2] 范世福，肖松山，赵友全，等.显微荧光光谱成像仪的研究与设计[J].仪器仪表学报，2005,26(10):1056-1059.FAN S F,XIAO S S,ZHAO Y Q,et al.Research and design of microscopic fluorescent spectral imager[J].Chinese Journal of Scientific Instrument,2005,26(10):1056-1059.(in Chinese)
- [3] YUN H Z,BIAN H,YA K D,et al.A new multichannel spectral imaging laser scanning confocal microscope[J].Computational and Mathematical Methods in Medicine,2013,2013:1-8.
- [4] 张运海，杨皓旻，孔晨晖.激光扫描共聚焦光谱成像系统[J].光学精密工程，2014,22(6):1446-1453.ZHANG Y H,YANG H M,KONG C H.Spectral imaging system on laser scanning confocal microscopy[J].Optics and Precision Engineering,2014,22(6):1446-1453.(in Chinese)
- [5] 齐敏珺，王新全，于翠荣，等.静态显微光谱成像系统的研制[J].光学精密工程，2015,23(5):1240-1245.QI M J,WANG X Q,YU C R,et al.Development of static microscopic spectral imaging system[J].Optics and Precision Engineering,2015,23(5):1240-1245.(in Chinese)
- [6] 廖乘胜，曾立波，吴琼水.一种多波段光源的显微多光谱成像系统研究[J].光子学报，2017,46(11):1130001.LIAO C S,ZENG L B,WU Q S.Reaserch of a microscopic spectral imaging system with multi-band illuminants[J].Acta Photonica Sinica,2017,46(11):1130001.(in Chinese)
- [7] 荣新，刘国超，栾弘义，等.二维材料的变温拉曼光谱研究[J].物理实验，2019,39(7):1-10.RONG X,LIU G C,LUAN H Y,et al.Temperature-dependent Raman spectra of 2D materials[J].Physics Experimentation,2019,39(7):1-10.(in Chinese)
- [8] 翟家振，张元龙，孔令杰.简易荧光显微镜的制作及其在生物医学中的应用[J].物理实验，2020,40(8):24-27.ZHAI J Z,ZHANG Y L,KONG L J.Fabrication and biomedical application of custom fluorescence microscope[J].Physics Experimentation,2020,40(8):24-27.(in Chinese)
- [9] 张朝晖.发展研究型基础物理实验教学的理念与实践[J].物理实验，2021,41(7):37-41.ZHANG Z H.Ideas and practice on development of research-type teaching of the basic physics experiments[J].Physics Experimentation,2021,41(7):37-41.(in Chinese)
- [10] 赵伟，王中平，韦先涛，等.拔尖人才培养背景下开展英才物理实验教学试点的总结和展望[J].物理实验，2021,41(9):23-28.ZHAO W,WANG Z P,WEI X T,et al.Pilot teaching of elite physics experiment under the background of top-notch talent training[J].Physics Experimentation,2021,41(9):23-28.(in Chinese)
- [11] 孙腊珍，张增明.研究型大学物理实验教学平台建设的实践与探索[J].实验技术与管理，2008,25(10):18-20.SUN L Z,ZHANG Z M.Practice and exploration on construction for platform of experimental teaching in research universities[J].Experimental Technology and Management,2008,25(10):18-20.(in Chinese)
- [12] 刘东奇.荧光显微镜自组实验设计[J].物理实验，2018,38(9):21-23.Liu D Q.Experimental design of self-assembled fluorescence microscope[J].Physics experimentation,2018,38(9):21-23.(in Chinese)
- [13] 刘东奇，孔勇发.显微光谱成像装置研制与实验设计[J].实验技术与管理，2020,37(2):104-106.Liu D Q,Kong Y F.Development and experimental design of microscopic spectral imaging device[J].Experimental Technology and Management,2020,37(2):104-106.(in Chinese)
- [14] YAN T,QIAO X,LIU X,et al.Photoluminescence properties and exciton dynamics in monolayer WSe2[J].Appl.Phys.Lett.,2014,105:101901.
- [15] ROSS J S,KLEMENT P,JONES A M,et al.Electrically tunable excitonic light-emitting diodes based on monolayer WSe2 p-n junctions[J].Nat.Nanotech.,2014,9:268-272.
- [16] LIU D Q,YAN X Q,GUO H W,et al.Substrate effect on the photoluminescence of chemical vapor deposition transferred monolayer WSe2[J].J.Appl.Phys.,2020,128:043101.
- [17] PARK S,MUTZ N,SCHULTZ T,et al.Direct determination of monolayer MoS2 and WSe2 exciton binding energies on insulating and metallic substrates[J].2D Mater.,2018,5:025003.